Tweet
Prepping a sample for a GS Jr run. Realized the Enrich and Seq primers got left out overnight at room temperature. We've got calls (and emails) in to Roche tech support, but it's been more than an hour, and now we're just sitting here twiddling our thumbs. Anyone know if these reagents are ruined? Or are they like real primers and likely fine?
-
-
I don't have any direct experience with your issue, but I suspect they will be just fine. At this point, you have nothing to lose by going ahead with the enrichment. If the enrichment primer has gone bad, you will get poor results from your enrichment and it will be clear after the washes. If the enrichment primer is okay then the sequencing primer should be fine as well. I'd say go ahead try it, then come back here and let us know how it worked out. -
So....how did it turn out? Did they still work?Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment