Hi everyone,
I am an absolute novice to chip-seq and I have to plan a chip-seq experimnent involving the overexpression of a tanscription factor in microvascular endothelial cells from the skin and or lungs. So I have two conditions, control untreated and TF-overexpressed conditions. I plan to use 4 biological replicates per condition. It will be dificult to generate sufficient cell amounts from cells isolated from patients, so I want to purchase immortalised cell lines.
I wish to find out if using different cell lines of the same cell type from the same tissue is similar to having cells from different patients.
I will be very grateful for any clarification and also any link thank can help me plan my chip-seq experiement better.
Thank you in advance for your Kind help.
I am an absolute novice to chip-seq and I have to plan a chip-seq experimnent involving the overexpression of a tanscription factor in microvascular endothelial cells from the skin and or lungs. So I have two conditions, control untreated and TF-overexpressed conditions. I plan to use 4 biological replicates per condition. It will be dificult to generate sufficient cell amounts from cells isolated from patients, so I want to purchase immortalised cell lines.
I wish to find out if using different cell lines of the same cell type from the same tissue is similar to having cells from different patients.
I will be very grateful for any clarification and also any link thank can help me plan my chip-seq experiement better.
Thank you in advance for your Kind help.
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