I've been looking into the Illumina TST170 sample prep kit. Reading the protocol, you either make a sheared DNA library or a cDNA library from RNA, then both go into the hybridisation capture based. But the data sheet says that fusions and splice variants can only be detected from RNA for a subset of the 170 genes. Why is this, if they are all going into the same capture?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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