Hi!
I have a question regarding my small-RNA library results on my bioanalyzer.
Recording to the scriptminer protocol you should get a peak around 150 bp (including adapters and miRNA (18-40 nt).
Attached you find my bioanalyzer results on CD4 cells (RIN 9.0)
Library 1 is totRNA from manufacturer. THis looks just like it should be. The 3 next libraries is my CD4 human cells. Here i have DNA around 150 bp, but i also got a peak around 200 bp. Is this normal? Is it just larger rna fragments in these type of cells?
I also did a small-RNA library prep in wheat cells. Here my results showed a peak that had an area from 150 bp- 300 bp) Should i just go further with the rest of my samples?
I have a question regarding my small-RNA library results on my bioanalyzer.
Recording to the scriptminer protocol you should get a peak around 150 bp (including adapters and miRNA (18-40 nt).
Attached you find my bioanalyzer results on CD4 cells (RIN 9.0)
Library 1 is totRNA from manufacturer. THis looks just like it should be. The 3 next libraries is my CD4 human cells. Here i have DNA around 150 bp, but i also got a peak around 200 bp. Is this normal? Is it just larger rna fragments in these type of cells?
I also did a small-RNA library prep in wheat cells. Here my results showed a peak that had an area from 150 bp- 300 bp) Should i just go further with the rest of my samples?
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