We recently have problems with 16S runs. We have been following the illumina protocol for v3-v4 regions, but have difficulties to get the optimum cluster density and >Q30 values.
We multiplexed 96 samples with Nextera XT v2 set A. Loaded 4pM lib+20% 4pM PhiX.
Now we see another problem, after 25 cycles there was values for cluster density 507K/mm2, but no values for Clusters PF and >Q30.
Is there anyone had the same problem? There was no error or warning message, the run is still going on.
We multiplexed 96 samples with Nextera XT v2 set A. Loaded 4pM lib+20% 4pM PhiX.
Now we see another problem, after 25 cycles there was values for cluster density 507K/mm2, but no values for Clusters PF and >Q30.
Is there anyone had the same problem? There was no error or warning message, the run is still going on.
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