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  • htseq-count ERROR in GFF File?

    Hello guys,

    I'm trying to do a summary of counts in usegalaxy.org. I convert Fatq groomer to sam with Map with BWA for Illumina (Galaxy Version 1.2.3) with a camponotus floridanus genome and
    now i have a sam file:

    ~98,000,000 lines, 24,027 comments
    format: sam database?
    BWA Version: 0.5.9-r16 BWA run on single-end data



    And a gff3 file from http://hymenopteragenome.org/campono...tium_datasets:

    ~190,000 lines
    format: gff3 database?
    uploaded gff3 file

    When i do a summary of counts with htseq-count:

    Fatal error: Unknown error occured
    Error occured when processing GFF file (line 5 of file /galaxy-repl/main/files/016/000/dataset_16000531.dat):
    Feature CFLO10001-RA does not contain a 'gene_id' attribute
    [Exception type: ValueError, raised in count.


    Thanks you everyone!

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