Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Tru Seq-Very faint bands of small RNA library jaymiRNA Sample Prep / Library Generation 8 09-20-2012 08:58 AM
aDNA & Library prep xanqueta Sample Prep / Library Generation 6 02-23-2012 06:29 AM
Does non-Y-shaped adapter work for Illumina library? skblazer Sample Prep / Library Generation 12 10-27-2011 07:06 PM
Rapid Library Adapter Dimer Br3ndan Sample Prep / Library Generation 1 05-05-2011 05:36 AM
titrate adapter and others xenia.zhang Illumina/Solexa 0 01-13-2010 08:04 AM

Thread Tools
Old 01-13-2010, 10:55 AM   #1
Location: ma

Join Date: Jan 2010
Posts: 10
Default titrate adapter & extra bands in library

Hi guys,

this is my first thread here, and really appreciate any input from you. Sorry for posting this again here. It seems to fit here better than in sequencing techniques part.

I wonder how you guys titrate adapter to match DNA material amount before ligation? (SE library construction of illumina)

Coz the volume at this stage is only 10ul and the total amount of DNA after many steps of purification is probably only a couple ng. At this low concentration, how do you guys manage to titrate?

We noticed varying amount of fragments at size around 58bp and 82bp in our illumina library. What do you guys think these are? People have been saying that adapter dimer is around 120bp and primer dimer 80-85bp. But how come adapter dimer being 120bp, with each adapter being only 33-34bp? And for primer dimer, 58bp(primer 1.1)+34bp(primer 2.1) is not 80-85bp either.

Thanks for your help!

xenia.zhang is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:12 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO