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Old 01-17-2011, 11:48 AM   #1
menglv
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Default custom primer

I know only a little about Illumina's sequencing. Is it possible to use custom primers in Illumina's flow channel? Anybody know how their primer is attached to the polyacrylamide coating?
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Old 01-18-2011, 11:07 AM   #2
GERALD
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It isn't too hard. It's best to just make your oligos as similar as possible to Illumina's own. You should probably refer to this earlier thread on how to design the primers and adapters:
http://seqanswers.com/forums/showthread.php?t=198
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Old 01-18-2011, 01:46 PM   #3
HESmith
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I think the question is whether the oligos attached to the flow cell can be customized. Unfortunately, that is not possible.
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Old 01-18-2011, 02:01 PM   #4
GERALD
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Default Flowcell

I looked at Illumina's method and it seems like a big pain, especially if you're not Illumina. I bet Illumina wouldn't like it either. Let us know if you're ever successful.
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