Go Back   SEQanswers > Sequencing Technologies/Companies > 454 Pyrosequencing
Similar Threads
Thread Thread Starter Forum Replies Last Post
Roche 454 DataProcessing CentOS denvaar 454 Pyrosequencing 0 02-09-2012 03:22 PM
roche 454 adapter filtering ikim Bioinformatics 0 12-02-2010 03:06 PM
Roche 454 REMe user ChristianBourne 454 Pyrosequencing 1 10-11-2010 10:21 AM
Roche Junior amplicon adapters jffkm General 0 02-11-2010 02:22 PM
non paired adapters in 454 data donniemarco 454 Pyrosequencing 3 10-19-2009 09:33 AM

Thread Tools
Old 01-26-2012, 08:48 AM   #1
Junior Member
Location: Glasgow

Join Date: Dec 2011
Posts: 3
Default Roche 454 RL adapters.. help!!!


We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT ( can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul is offline   Reply With Quote
Old 01-26-2012, 02:57 PM   #2
Senior Member
Location: Australia

Join Date: May 2009
Posts: 155

This technical bulletin should help answer your questions.
Attached Files
File Type: pdf TCB-10010_MIDAdaptorsforRapidLibraryPreparations.pdf (426.1 KB, 149 views)
RCJK is offline   Reply With Quote
Old 01-27-2012, 02:39 AM   #3
Junior Member
Location: Glasgow

Join Date: Dec 2011
Posts: 3

Dear RCJK, many thanks for the technical bulletin.
Vartul is offline   Reply With Quote
Old 09-11-2020, 08:23 AM   #4
kevin zhang
Junior Member
Location: China

Join Date: Sep 2020
Posts: 3
Post please give me this technical bulletin

Originally Posted by Vartul View Post
Dear RCJK, many thanks for the technical bulletin.
Dear sir, can you give me this technical bulletin?thank you so much.
kevin zhang is offline   Reply With Quote
Old 01-14-2021, 12:06 AM   #5
Junior Member
Location: greece

Join Date: Jan 2021
Posts: 2

could you upload this technical bulletin again?
I cannot download it from the link you provided already a decade ago...
ellli555 is offline   Reply With Quote

454, adaptors, roche

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:14 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO