ChIP-Seq: Single-tube linear DNA amplification for genome-wide studies using a few th

Newsbot! · 01-28-2012, 02:00 AM

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Single-tube linear DNA amplification for genome-wide studies using a few thousand cells.

Nat Protoc. 2012;7(2):328-38

Authors: Shankaranarayanan P, Mendoza-Parra MA, van Gool W, Trindade LM, Gronemeyer H

Abstract
Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (

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01-28-2012, 02:00 AM	#1
Newsbot! RSS Posting Maniac Join Date: Feb 2008 Posts: 1,443	ChIP-Seq: Single-tube linear DNA amplification for genome-wide studies using a few th Syndicated from PubMed RSS Feeds Single-tube linear DNA amplification for genome-wide studies using a few thousand cells. Nat Protoc. 2012;7(2):328-38 Authors: Shankaranarayanan P, Mendoza-Parra MA, van Gool W, Trindade LM, Gronemeyer H Abstract Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (