Innovated clonal amplification

Michael L. Altshuler · 12-17-2013, 03:26 AM

Hi Colleagues,

I wonder what is the difference between Avalanche and Wildfire methods of generation clones from single molecules? They seem to be synonyms.

Michael L. Altshuler · 12-18-2013, 10:50 AM

Sorry, I have forgotten that Wildfire is matched to Solid instruments and has been available for some time.

Dornfield · 10-02-2017, 01:00 AM

I realise that this is replying to a very old Thread, but my understanding is that Wildfire was the clonal amplification technology applied to SOLiD, whereas Avalanche was the (same) technology applied on the Ion Torrent platform.

From what I can see, it all went very quiet with these clonal amp technologies several years ago: have they died the death? I recall seeing some user* application of Wildfire on the SOLiD 5500W - with the W presumably being 'Wildfire'? - *Audrey Papp, The Ohio State University.

seqitall · 12-02-2017, 01:41 PM

Hi - Any chance someone has raw images (i.e. tiffs) from a 5500W? Curious to learn more about cluster size, shape, intensities from template walking.

Thanks!

Giovanni20 · 09-14-2021, 12:01 AM

Quote:

Originally Posted by Dornfield

I realize that this is replying to a very old Thread, but my understanding is that Wildfire was the clonal amplification technology applied to SOLiD, whereas Avalanche was the (same) technology applied on the Ion Torrent platform.

From what I can see, it all went very quiet with these clonal amp technologies several years ago: have they died the death? I recall seeing some user* application of Wildfire on the SOLiD 5500W - with the W presumably being 'Wildfire'? - *Audrey Papp, The Ohio State University.

Cluster shape and intensities were super.

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12-17-2013, 03:26 AM	#1
Michael L. Altshuler Member Location: Moscow Join Date: Nov 2007 Posts: 13	Innovated clonal amplification Hi Colleagues, I wonder what is the difference between Avalanche and Wildfire methods of generation clones from single molecules? They seem to be synonyms.

12-18-2013, 10:50 AM	#2
Michael L. Altshuler Member Location: Moscow Join Date: Nov 2007 Posts: 13	Sorry, I have forgotten that Wildfire is matched to Solid instruments and has been available for some time.

10-02-2017, 01:00 AM	#3
Dornfield Member Location: Oxford Join Date: Aug 2010 Posts: 11	I realise that this is replying to a very old Thread, but my understanding is that Wildfire was the clonal amplification technology applied to SOLiD, whereas Avalanche was the (same) technology applied on the Ion Torrent platform. From what I can see, it all went very quiet with these clonal amp technologies several years ago: have they died the death? I recall seeing some user* application of Wildfire on the SOLiD 5500W - with the W presumably being 'Wildfire'? - *Audrey Papp, The Ohio State University.

12-02-2017, 01:41 PM	#4
seqitall Junior Member Location: California Join Date: Dec 2017 Posts: 2	Hi - Any chance someone has raw images (i.e. tiffs) from a 5500W? Curious to learn more about cluster size, shape, intensities from template walking. Thanks!