Go Back   SEQanswers > Literature Watch
Similar Threads
Thread Thread Starter Forum Replies Last Post
ChIP-Seq: Single-tube linear DNA amplification for genome-wide studies using a few th Newsbot! Literature Watch 0 01-28-2012 02:00 AM
ChIP-Seq: High-resolution genome-wide in vivo footprinting of diverse transcription f Newsbot! Literature Watch 1 08-24-2011 07:45 AM
ChIP-Seq: High-Resolution Genome-wide Mapping of the Primary Structure of Chromatin. Newsbot! Literature Watch 0 01-19-2011 08:30 AM
ChIP-Seq: Data structures and compression algorithms for high-throughput sequencing t Newsbot! Literature Watch 0 10-16-2010 02:00 AM
ChIP-Seq: Savant: Genome Browser for High Throughput Sequencing Data. Newsbot! Literature Watch 0 06-22-2010 02:00 AM

Thread Tools
Old 10-16-2010, 02:00 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default ChIP-Seq: ChIP-Seq Using High-Throughput DNA Sequencing for Genome-Wide Identificatio

Syndicated from PubMed RSS Feeds

ChIP-Seq Using High-Throughput DNA Sequencing for Genome-Wide Identification of Transcription Factor Binding Sites.

Methods Enzymol. 2010;470:77-104

Authors: Lefrançois P, Zheng W, Snyder M

Much of eukaryotic gene regulation is mediated by binding of transcription factors near or within their target genes. Transcription factor binding sites (TFBS) are often identified globally using chromatin immunoprecipitation (ChIP) in which specific protein-DNA interactions are isolated using an antibody against the factor of interest. Coupling ChIP with high-throughput DNA sequencing allows identification of TFBS in a direct, unbiased fashion; this technique is termed ChIP-Sequencing (ChIP-Seq). In this chapter, we describe the yeast ChIP-Seq procedure, including the protocols for ChIP, input DNA preparation, and Illumina DNA sequencing library preparation. Descriptions of Illumina sequencing and data processing and analysis are also included. The use of multiplex short-read sequencing (i.e., barcoding) enables the analysis of many ChIP samples simultaneously, which is especially valuable for organisms with small genomes such as yeast.

PMID: 20946807 [PubMed - in process]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:49 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO