I'm about to start running 100s of libs through double spri cleaning. In the past I've used ampure and made my own PEG solution which allowed me to use ~1/5 the recommended amount of Ampure. (single clean up for ~$0.25). This would mean $0.50 double clean ups which isn't unbearable but since I'm starting at the beginning for optimizing this protocol (and we don't use Ampure for any other lib preps) I feel like this is the time to look around for cheaper ways to do it. I'm hesitant to go fully homebrew because we're a core facility and I worry about clients balking at us not using a kit.
So please anyone who does large numbers of preps-how are you controlling bead costs?
So please anyone who does large numbers of preps-how are you controlling bead costs?
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