Rolling circle dsDNA vs ssDNA experience?

hoytpr · 11-20-2018, 08:32 AM

Does anyone have experience with samples using rolling circle amplification that can tell me whether they have relatively high or low amounts of ssDNA? The client is not local so it would help to know in advance whether they need to ship much more based on A260 readings before starting library preps.
Thanks,
-p

GW_OK · 12-13-2018, 11:41 AM

Comparing A260 vs picogreen/Qubit should tell you if there's a large amount of ssDNA present.

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11-20-2018, 08:32 AM	#1
hoytpr Member Location: Stillwater Join Date: Dec 2009 Posts: 62	Rolling circle dsDNA vs ssDNA experience? Does anyone have experience with samples using rolling circle amplification that can tell me whether they have relatively high or low amounts of ssDNA? The client is not local so it would help to know in advance whether they need to ship much more based on A260 readings before starting library preps. Thanks, -p

12-13-2018, 11:41 AM	#2
GW_OK Senior Member Location: Oklahoma Join Date: Sep 2009 Posts: 411	Comparing A260 vs picogreen/Qubit should tell you if there's a large amount of ssDNA present.