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Old 11-20-2018, 08:32 AM   #1
Location: Stillwater

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Default Rolling circle dsDNA vs ssDNA experience?

Does anyone have experience with samples using rolling circle amplification that can tell me whether they have relatively high or low amounts of ssDNA? The client is not local so it would help to know in advance whether they need to ship much more based on A260 readings before starting library preps.
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Old 12-13-2018, 11:41 AM   #2
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Location: Oklahoma

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Comparing A260 vs picogreen/Qubit should tell you if there's a large amount of ssDNA present.
GW_OK is offline   Reply With Quote

rca, rolling circle

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