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  • Interpreation of RSEQC read_distribution.py results

    Dear Seqanswers List,

    Here is my ouput from read_distribution.py from RSEQC run from Galaxy

    Total Reads 54875279
    Total Tags 58170116
    Total Assigned Tags 54031503
    =====================================================================
    Group Total_bases Tag_count Tags/Kb
    CDS_Exons 39184887 10217883 260.76
    5'UTR_Exons 37214570 10963357 294.60
    3'UTR_Exons 58010731 4386101 75.61
    Introns 1539911688 6018229 3.91
    TSS_up_1kb 24518972 906468 36.97
    TSS_up_5kb 110212503 4956374 44.97
    TSS_up_10kb 201827137 13004223 64.43
    TES_down_1kb 27333233 59583 2.18
    TES_down_5kb 118264548 3689226 31.19
    TES_down_10kb 210002921 9441710 44.96

    My questions:
    Where does rRNA fit in this picture?
    What about other transcripts other than mRNA such as pseudogenes?

    Thanks and best wishes,
    Rich
    Richard Friedman,
    Columbia University

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