Tweet
I am working on a workflow for a hybrid denovo assembly of 454 and illumina data using the CLC genomics workbench. I was wondering if anyone has done similar work, and if so what steps they took to get optimal results.
Thanks
Thanks
-
-
Dear BardJ. Sorry about the late answer. I was just splunking through SeqAnswers, and I found your question. Hybrid assemblies are a common use of the Workbench. I can send you a publication that references this work. In general, there are separate assembly criteria for each type of data, and the default criteria are a good place to start. Since you posted, the de novo assembler is even more efficient. I hope you gave it a try. If not, try it again now.
Best,
Naomi
(employee of CLC)
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment