emPCR SV Transpette?

[Anthony.287]

Hi all,
I've been working with the SV emPCR kits a lot lately, and I've noticed that a 200uL tip fits the tubing for the MV and LV transpette quite nicely. I'd like to make a single channel transpette; I'd need the tubing and the 50ml caps, which I bet I can find for less cost than buying the transpettes from Roche and throwing half of them away. Has anyone done this? Does anyone know where I can find 50ml caps with attachments for tubing? These syringes are getting old...
Thanks!

[HMorrison]

We haven't bought breaking kits in a long time now--after the first try with the transpettes, I started cutting them off and using a single 1 ml Rainin tip. Transpette was too sloppy. We searched for a supplier of the caps with tubing attachments but couldn't find any and Roche wouldn't provide information on their source. So we now buy tubing and wash and reuse the caps. I use the same setup for SVs.

[Anthony.287]

That's perfect! Thank you!
I'm so glad I read this while processing 16 syringes..

[Anthony.287]

Where do you get the tubing, and what size is it, if you don't mind my asking?

[HMorrison]

We get it from our institution's stockroom, but it seems to be Excelon RNT tubing, 1/8th inch ID, 1/4 OD, 1/16th wall purchased from US Plastic Corporation; www.usplastic.com.

[Anthony.287]

Excellent!
Thank you!

[Anthony.287]

One more question - how do you do the rest of the protocol? There are a few small differences downstream from the bead recovery, like the EtOH step in the LV and MV protocols, that aren't part of the SV. I would imagine that following the LV or MV protocol through to the end, using the correct bead numbers and adjusted volumes of course, would be fine. I'm just curious how this works for you.
Thanks again!

[HMorrison]

One more question - how do you do the rest of the protocol? There are a few small differences downstream from the bead recovery, like the EtOH step in the LV and MV protocols, that aren't part of the SV. I would imagine that following the LV or MV protocol through to the end, using the correct bead numbers and adjusted volumes of course, would be fine. I'm just curious how this works for you.
Thanks again!

Sorry for the slow response--yes, we follow the LV protocol. I've never even looked at MV version.