I'm currently using Nextera XT to prepare libraries for MiSeq. This involves 'tagmentation' which simultaneously fragments (enzymatically) and adds a tag sequence. We then amplify using Nextera XT indices.
My question is - if we were to fragment our dsDNA by sonication, how would we then amplify the fragments using Nextera XT indices? I assume the tag sequence is essential - is there any way around this?
Thanks in advance for any advice!
My question is - if we were to fragment our dsDNA by sonication, how would we then amplify the fragments using Nextera XT indices? I assume the tag sequence is essential - is there any way around this?
Thanks in advance for any advice!
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