I have a genome library at 350bp. I sequenced it using 2x150bp read length on Miseq v2. Will I miss out on information because the insert size (350bp) is larger than the total sequencing read length (300bp). Should I always make the insert size smaller than the read length? What if I wanted to run a 2x75bp read length , how much will it sequence of the 300bp insert size?
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The truth is that reads are overlapped, so the missed part of this read pair should be revealed by another one. Besides, a library with insert size of 350bp should have a size around 350, may be 250~450 sometimes. furthermore, sequencing 250 bp insertion with 2*150bp, I would like to say it's a waste of data, cause there are 50 bp is sequence twice.
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Will I miss out on information because the insert size (350bp) is larger than the total sequencing read length (300bp).
Should I always make the insert size smaller than the read length?
What if I wanted to run a 2x75bp read length , how much will it sequence of the 300bp insert size?
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