Hi,
I am using casava 1.8.2 for bcl to fastq conversion. I have few questions:
The command I use is:
/usr/local/bin/configureBclToFastq.pl --input-dir /usr/local/share/CASAVA-1.8.2/examples/Validation/110120_P20_0993_A805CKABXX/Data/Intensities/BaseCalls --output-dir Unaligned
I get the output and I can identify each sample separately without giving samplesheet.csv file
Questiotion 1) Is the samplesheet.csv necessary for the conversion? If we do not provide samplesheet, will it have any impact on the output?
Question 2) I am getting different numbers of fastq files for different samples like 10 files (5 for read 1 and 5 for read 2) for sample 1, 14 (7 for each read) for sample 2 and so on. Why is it like this? Is there any way that I can get a single file for each sample?
I am using casava 1.8.2 for bcl to fastq conversion. I have few questions:
The command I use is:
/usr/local/bin/configureBclToFastq.pl --input-dir /usr/local/share/CASAVA-1.8.2/examples/Validation/110120_P20_0993_A805CKABXX/Data/Intensities/BaseCalls --output-dir Unaligned
I get the output and I can identify each sample separately without giving samplesheet.csv file
Questiotion 1) Is the samplesheet.csv necessary for the conversion? If we do not provide samplesheet, will it have any impact on the output?
Question 2) I am getting different numbers of fastq files for different samples like 10 files (5 for read 1 and 5 for read 2) for sample 1, 14 (7 for each read) for sample 2 and so on. Why is it like this? Is there any way that I can get a single file for each sample?
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