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  • D. Melanogaster ChIP

    I recently tried to do a ChIP-like experiment on chromatin from drosophila S2 cells and found that a large portion of my ChIP reads, but also my input reads, aligned to repetitive elements. I have done this experiment in human cells before and not had this result. I wasn't sure if this was a hallmark of trying to do sequencing in Drosophila, or if it was just a problem with my particular assay.

    Has anyone seen a problem like this before?

    Is there anything I can change in my alignment (done using MACS), or in my analysis to avoid this?

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  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

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