Hi,
using Dynabeads mRNA purification kit (polyA, LifeTech), on Drosophila total RNA, we are always left with about 11% rRNA. Primarily 18s.
We have tried double and triple purification, re-using beads, using new beads; and with varying dilutions of the RNA and the beads.
The kit normally works beautifully on mouse and human RNA.
Can anybody pls tell us how make it work on Drosophila???
SbC
using Dynabeads mRNA purification kit (polyA, LifeTech), on Drosophila total RNA, we are always left with about 11% rRNA. Primarily 18s.
We have tried double and triple purification, re-using beads, using new beads; and with varying dilutions of the RNA and the beads.
The kit normally works beautifully on mouse and human RNA.
Can anybody pls tell us how make it work on Drosophila???
SbC
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