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  • Visualization of Transposon insertions

    Hi everyone,

    I have my transposon mutant library, and I want to visualize the mapped transposon insertions to the reference genome via DNAplotter (Artemis).

    I need the plot to be like the attached picture from the literature (where the red lines represent the transposon insertions).

    I could not do the same in Artemis because non of the output files from BioTradis pipeline are in EMBL format. I can only visualize BAM and insertion plot as shown in (My_entry). Therefore, could anyone help or suggest how can I convert the files that mapped to the reference genome (e.g., BAM, fq.gz) to EMBL in order to visualize the insertions in DNAplotter.

    Thanks in advance,
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