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Old 07-16-2012, 03:54 PM   #1
wdt
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Default DESeq high within group dispersion

Hi forum,

I am analyzing counts data from Tophat bam followed by HTSeq.
I have 11 biological replicates for group 1 and 10 biological replicates for group 2.

Looking at the attached dispersion plots, is it a fair interpretation that there
is too much within group dispersion (most values around 1) and within group dispersion is simply too large for anything called differentially expressed? I found ~150 genes with p<0.05 and none of the survived 0.1 FDR (giving padj=1 for all).

What'd would be a suggested way to analyze this data further?

Regards,

WDT
Attached Images
File Type: png MA.png (6.0 KB, 32 views)
File Type: png case_1.png (24.1 KB, 37 views)
File Type: png case_2.png (24.2 KB, 25 views)

Last edited by wdt; 07-16-2012 at 04:28 PM. Reason: typo correction
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