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Old 11-16-2009, 06:00 AM   #1
Meliss a
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Location: Ann Arbor, MI

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Default Frozen flow cell

Has anyone ever used a flow cell that has been frozen. We had a mishap with our refrigerator and my flow cells all froze!
Tech-support advised against using them but I was wondering if anyone has and what the outcome was.
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Old 11-16-2009, 12:48 PM   #2
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Location: Monash University, Melbourne, Australia.

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I haven't tried it, but I was told that the flowcells will delaminate if they're frozen, or even put on ice.

Let us know how it goes if you do try!
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Old 09-16-2014, 02:47 AM   #3
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Anyone? @Melissa did you found it? or in elsewhere?
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Old 09-14-2015, 12:49 AM   #4
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Our lab has the experience....>_<. We test run a Miseq flow cell that has been frozen for 3 weeks in its original buffer. We test run the 150 cycles reagent on it and it turns out to be fine in both cluster density and quality.
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Old 03-06-2019, 05:42 AM   #5
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We had accidentally frozen incorporation buffer and a nano flow cell for two weeks. Thawed both in water at room temperature and successfully sequenced with a 300 cycle kit.
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