Fragment length Illumina

CatVincent · 11-23-2010, 04:29 AM

1. Is there an upper size limit of fragment binding to the Illumina flow cells?
2. Would this be the same for Illumina GSII and for HS2000?
3. Can the Illumina protocol used for cluster formation be modified with respect to synthesis time?
4. If shorter fragments than the desired 1.5 kb get (unadvertedly) amplified too, would we obtain mixed clusters of short and long bridges that cannot be disentangled?
5. Did you ever try two or even more rounds of size selection by cutting from gels to avoid problems with shorter reads accumulating during cluster formation?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Cufflinks' estimated fragment length mean and standard deviation	d f	Bioinformatics	12	01-18-2014 10:32 AM
Illumina fragment length distribution	delphi_ote	Genomic Resequencing	3	05-18-2012 01:59 AM
Fragment length limits?	krobison	Ion Torrent	18	12-08-2011 10:49 AM
maximal fragment length GA?	remcoloos	Illumina/Solexa	1	04-14-2010 03:20 PM
Maximum fragment length	Jenny Russ	Illumina/Solexa	5	09-30-2008 03:55 PM

11-23-2010, 04:29 AM	#1
CatVincent Junior Member Location: Germany Join Date: Nov 2010 Posts: 2	Fragment length Illumina 1. Is there an upper size limit of fragment binding to the Illumina flow cells? 2. Would this be the same for Illumina GSII and for HS2000? 3. Can the Illumina protocol used for cluster formation be modified with respect to synthesis time? 4. If shorter fragments than the desired 1.5 kb get (unadvertedly) amplified too, would we obtain mixed clusters of short and long bridges that cannot be disentangled? 5. Did you ever try two or even more rounds of size selection by cutting from gels to avoid problems with shorter reads accumulating during cluster formation?