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Old 07-08-2013, 10:35 PM   #1
Location: Russia

Join Date: Dec 2007
Posts: 88
Default Chip washing station

Can I re-use the chips with low filling of wells?
genseq is offline   Reply With Quote
Old 01-09-2014, 06:13 PM   #2
Location: Virginia

Join Date: Jan 2014
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Not recommended.

The ISPs from the previous run would probably contribute signal to the subsequent run. I suppose you could take the x:y coordinates in the read IDs from the previous run and exclude those wells from the subsequent run with some custom scripts, but that's a hack that requires going around the Torrent Server to get a fastq from the bam, correct it, then re-analyze it offline. I'm willing to bet that my labor cost for that task would exceed the cost of the chip, even for a 318 or PI. But then again, I'm not a bioinformatician.

Ion chip loading…it's all in the wrist. Or the Chef.
andylemire is offline   Reply With Quote
Old 01-14-2014, 06:34 AM   #3
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I doubt you would get any signal from ISPs left over from the previous run. They will have double-stranded DNA on them whereas the new ISPs will have single-stranded DNA with primers annealed. If the DNA on the old ISPs isn't fully double-stranded (i.e. if the fragment was long enough that the polymerase didn't get to the end during the run), you may get signal from those wells, but that sequence is unlikely to start with the key sequence and will therefore filtered.
ajthomas is offline   Reply With Quote

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