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Old 09-27-2013, 06:29 AM   #81
thomasblomquist
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Huh??? That's backwards from my experience; did you mean "Ion" where you wrote "Illumina"?
Oops. Yes. Ion has the homopolymer issue.

Illumina is awesome data quality. Still we will keep Ion torrent for lower volume quicker turn around for more acute dx panels.
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Old 10-16-2013, 12:22 AM   #82
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Is Proton 2 chip a vaporware?
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Old 10-16-2013, 01:18 AM   #83
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Is Proton 2 chip a vaporware?
From what I have heard it is getting released soon, they say end of this year, so maybe Feb next year . But the reps are saying 40-50 Gb of sequence now instead of the 100 Gb sequence it was initially marketed as. And read lengths are still only 100 bp instead of the 400 that marketing was spouting. It is getting more and more disapointing (since my lab has a proton).
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Old 10-16-2013, 11:57 AM   #84
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From what I have heard it is getting released soon, they say end of this year, so maybe Feb next year . But the reps are saying 40-50 Gb of sequence now instead of the 100 Gb sequence it was initially marketed as. And read lengths are still only 100 bp instead of the 400 that marketing was spouting. It is getting more and more disapointing (since my lab has a proton).
I agree with Jeremy that this probably isn't 'vaporware', but it has definitely been delayed (10 months and counting...) In terms of output, Ion Torrent is now officially saying it will launch with 32Gb of output per run, climbing to 64Gb over a ~12 month period.
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Old 10-22-2013, 07:19 AM   #85
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Yeah IonTorrent is saying that for a year now. IonTorrent says a lot of things, but do they deliver? How is the Avalanche coming along?
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Old 10-22-2013, 08:33 AM   #86
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Yeah IonTorrent is saying that for a year now. IonTorrent says a lot of things, but do they deliver? How is the Avalanche coming along?
Ion Isothermal Amplification Chemistry (formerly known as Avalanche)

This new, single-tube chemistry delivers isothermal amplification of 500 bp library templates in as little as 30 minutes. The complete template preparation process takes just two hours, and will enable the industry's fastest benchtop sequencing workflow, by far.
- See more at: http://ioncommunity.lifetechnologies....BTJ8mb0N.dpuf
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Old 10-22-2013, 12:00 PM   #87
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Yeah IonTorrent is saying that for a year now. IonTorrent says a lot of things, but do they deliver? How is the Avalanche coming along?
It was announced today at Ion World that customers will get early access to Avalanche next month with general availability early next year.
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Old 10-23-2013, 01:54 AM   #88
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Good to see that they are kicking. Hope it all ends well and we see something good delivered in Q1
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Old 10-23-2013, 04:41 AM   #89
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I'll keep my fingers crossed! Good to see that the avalanche is (finally) becoming available..
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Old 12-30-2013, 12:39 AM   #90
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These are online presentation and i like so much..I seen many design studio but there is not for your presentation...thanks
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Old 01-07-2014, 09:07 PM   #91
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could post any links considering those avalanche news or presetnations?
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Old 01-28-2014, 04:08 PM   #92
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Can anyone update this question? We have a HiSeq2500 and a MiSeq here, and we have the opportunity to obtain an IonTorrent PGM essentially for free (we have to pay capital depreciation costs, but nothing else). Is taking this free* PGM even worth it considering we already have a MiSeq? Are there any applications for which the PGM shines over the MiSeq? Or will it just sit there because the MiSeq is as good as or better than the PGM across the board?

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Old 01-28-2014, 08:26 PM   #93
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Are there any applications for which the PGM shines over the MiSeq?
The PGM is known for speed, decent read lengths (up to 400b) and not dealing too well with homopolymers. There's new chemistry on the horizon that might significantly improve the homopolymer issue.

Should you get one (more or less for free)? If you highly value quick runs (<1 day instead of 24 hours) and have lots of small targeted sequencing projects, it might be a good choice to add to your panel of instruments.

But nothing is truly free - be sure you have the time both to teach yourself how to use this new platform and to support it/keep it running.
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Old 01-28-2014, 09:21 PM   #94
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The PGM is known for speed, decent read lengths (up to 400b) and not dealing too well with homopolymers. There's new chemistry on the horizon that might significantly improve the homopolymer issue.

Should you get one (more or less for free)? If you highly value quick runs (<1 day instead of 24 hours) and have lots of small targeted sequencing projects, it might be a good choice to add to your panel of instruments.

But nothing is truly free - be sure you have the time both to teach yourself how to use this new platform and to support it/keep it running.
Thank you for your quick answer -- it's very much in line with my thinking. One of our primary concerns was about support and maintenance costs (rather than monetary costs). We do a fair amount of amplicon sequencing but our MiSeq isn't overbooked. We will likely be doing more of it in the near future, and potentially in GC-rich microbes, so the homopolymer issue was a concern too (and was one of the reasons I was turned off by the 454 data I analyzed in grad school -- too many indels -- though I realize things are always improving). Sounds like we'll really have to think this out. Do you have any references on the new chemistry? I'm all up-to-date with Illumina's R&D efforts but haven't heard much from Life. Thanks again!
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Old 01-28-2014, 09:42 PM   #95
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Happy to help. The new chemistry is called Hi-Q. An early access program for it was announced at ASHG, with a full launch expected around July this year. In theory it's already available to PGM customers (via the early access program), but I haven't heard much about it since the original announcement. You can read more about it here: http://ir.lifetechnologies.com/relea...leaseID=798937
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Old 01-28-2014, 09:52 PM   #96
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Quote:
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Happy to help. The new chemistry is called Hi-Q. An early access program for it was announced at ASHG, with a full launch expected around July this year. In theory it's already available to PGM customers (via the early access program), but I haven't heard much about it since the original announcement. You can read more about it here: http://ir.lifetechnologies.com/relea...leaseID=798937
Boom! Thanks again! I'm guessing we'll have another update come AGBT next month (unfortunately I'm on the waiting list...).
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Old 10-06-2014, 08:45 PM   #97
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Any updated answer to my question posted a year ago???

Is Proton 2 chip a vaporware?
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Old 10-06-2014, 08:53 PM   #98
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Any updated answer to my question posted a year ago???

Is Proton 2 chip a vaporware?
Well, the latest is 'early access in early 2015'. Probably not vaporware, but definitely not the "HiSeq killer" it was supposed to be. Too little, too late.
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Old 10-06-2014, 10:57 PM   #99
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lets see what comes new from IT on ASHG, I know Illumina will come with some more things but all of them have ben announced previously. Last thing i heard from a customer was that P2 will be around 20Gb of output so way below a killer for a Hiseq, NextSeq or a MiSeq.

Much more interesting is the bench top from CG/BGI and whatever Quiagen will release at some point for the clinical sector but also there i have no news (maybe All Seq has?? )
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Old 10-06-2014, 11:03 PM   #100
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Pyrosequencer BIGIS-4 (made in China):
http://english.big.cas.cn/ic/ip/2012...924_91378.html
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