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Old 10-20-2012, 01:41 AM   #1
Location: germany

Join Date: Apr 2010
Posts: 31
Default Dispertion RNAseq cummeRbund

Dear all,
I have anaylsed a RNAseq dataset with triplicates and 2 conditionsat the level of differential gene expression with HTSeq and this worked well (make biological sens and candidates confirmed by other technic). So I am confident that dataset is ok.
I now try to do the analyis at the level of transcipts with DExseq and cuffdiff.
I visualise the data from cuffdiff with cummeRbund and her is the grah for dispertion. It looks like that dispersion is higher for genes than isoforms. Could someone comment onto this?
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