SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
CummeRbund error NRiddiford RNA Sequencing 5 07-26-2013 11:18 AM
another CummeRbund question godzilla07 Bioinformatics 2 08-08-2012 08:22 PM
cummeRbund installation m_elena_bioinfo Bioinformatics 3 08-02-2012 01:42 AM
Total RNAseq and cufflinks/cuffmerge/cuffdiff/cummeRbund pipeline JWC Bioinformatics 8 08-01-2012 05:41 AM
who coined RNAseq? RNAseq as an alignment first approach only brachysclereid Bioinformatics 3 01-10-2012 12:17 PM

Reply
 
Thread Tools
Old 10-20-2012, 01:41 AM   #1
oliviera
Member
 
Location: germany

Join Date: Apr 2010
Posts: 31
Default Dispertion RNAseq cummeRbund

Dear all,
I have anaylsed a RNAseq dataset with triplicates and 2 conditionsat the level of differential gene expression with HTSeq and this worked well (make biological sens and candidates confirmed by other technic). So I am confident that dataset is ok.
I now try to do the analyis at the level of transcipts with DExseq and cuffdiff.
I visualise the data from cuffdiff with cummeRbund and her is the grah for dispertion. It looks like that dispersion is higher for genes than isoforms. Could someone comment onto this?
Attached Images
File Type: jpg Untitled-1.jpg (62.2 KB, 23 views)
oliviera is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:09 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO