SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
MinElute columns for TruSeq preps? bbeitzel Sample Prep / Library Generation 10 05-11-2012 04:46 AM
Correct kit for qPCR on Illumina preps? Heisman Sample Prep / Library Generation 2 06-08-2011 07:34 AM
Results with mate-pair preps dzerbino Illumina/Solexa 8 05-21-2011 12:02 PM
SPRI-TE Illumina library preps GW_OK Sample Prep / Library Generation 5 08-23-2010 09:13 AM
Ligase for DIY GAII library preps nickb Sample Prep / Library Generation 4 03-05-2010 10:36 AM

Reply
 
Thread Tools
Old 12-06-2013, 06:58 AM   #1
emolinari
Member
 
Location: New haven

Join Date: May 2013
Posts: 47
Default different cDNA preps

Hi All!
does anyone have any experience in running Nugen FFPE or Ovation V2 systems?
I am wondering if I need more seq depth compared to polyA selected RNA to get a somehow comparable library complexity.
I generally run 75bp, PE, 30 mil reads polyA-selected samples...

Any feedback is appreciated!!!
thanks
Manu
emolinari is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:07 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO