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ChIP-Seq mouse embryonic sample prep vishalmenon90 Sample Prep / Library Generation 2 10-04-2013 09:57 AM

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Old 06-15-2016, 02:51 PM   #1
shldbcrzy1
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Default Mouse brain tissue prep for ChiP seq

I am trying to prepare samples for down stream ChiP seq. The problem is that I am using mouse tissue and have trouble preparing the samples as there are few protocols for fresh tissue. My question's are
1. Do we fix samples before i homogenize the tissue and how
2. I have used covaris for chromatin shearing but when i am not sure if the SDS in the buffer will affect my downstream sequencing. Without SDS in my shearing buffer (which is same as the covaris kit) i do not see shearing.
3. What kit can i use for preraring the library if my starting material is too low and the samples further go through sorting to get special neuronal cells.

Thanks in advance for the help.
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