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  • Newbie: Primer with index

    Hi everyone, kinda new to the whole sequencing. So I want to run multiple sample in minion. There is barcode provided by oxford nanopore, but i also would like to attach index to my pcr.

    I am planning to make 18 or 22 bp of index.
    The question is, is there any platform that you use to design index? or is it just randomly decided by you?

  • #2
    You could just add the index to the primers when you order them.

    or

    SQK-RPB004 or SQK-PBK004 depending on your experiment.

    Comment


    • #3
      hmm, not really like that. So beside the one provided by nanopore, I plan to attach some kind of index to the primer itself, so the result can be divided further in one barcode.

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