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Old 02-06-2020, 09:49 AM   #1
Jieshu
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Location: ON, Canada

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Default Urgent: ATAC-seq barcoded primers

Hi guys,

I am new to ATAC-seq, I plan to try this method with Arabidopsis. I am going to order those primers from Supplementary Table 1 (Buenrostro et al., Nat Methods, 2013). For Ad1_noMX and bar-coded primers Ad2.1/2/3..., just order them as what they are? is there any modification for these bar-coded primers? I plan to order them with DESALT from Eurofins, could anyone give me some suggestions?

Thanks,

Jie
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Old 02-06-2020, 08:22 PM   #2
luc
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I believe you can use standard barcoded Nextera primers instead. Preferentially UDI dual indexed versions of the Nextera primers.
Simple desalted oligos will be fine indeed.
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