Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • New RNA-Seq library prep kit

    Epicentre Biotechnologies announces the availability of the new ScriptSeq(TM) mRNA-Seq Library Preparation Kit (Illumina-compatible). The kit uses a unique, patented terminal-tagging technology that simplifies strand-specific tagging and the preparation of directional mRNA-Seq libraries from rRNA-depleted or poly(A) RNA.

    Starting from just 50 ng of rRNA-depleted mRNA, or poly(A) RNA, the ScriptSeq kit produces adaptor-tagged libraries in about 3 hours without the need for end-repair, adaptor ligations, shearing the cDNA, or gel electrophoresis.

    For more information, please see the product page.
    Connect with Epicentre: Facebook | Twitter

  • #2
    Do you have any information on how this would perform for whole transcriptome analysis and not just mRNA-seq?

    Comment


    • #3
      The kit is actually suitable for whole-transcriptome analysis, since it uses random-primed cDNA synthesis. The choice of mRNA-Seq or WT would be determined by whether you were using poly(A)-enriched or rRNA-depleted RNA, respectively.
      Connect with Epicentre: Facebook | Twitter

      Comment


      • #4
        Hi - I was wondering if your adapters were paired-end ready for the mRNA seq kit, and if your adapters are computable with Illumina's indexes and multiplexing pcr primers.

        Comment


        • #5
          Yes, the adaptors are designed to enable paired-end reads. They are also suitable for multiplexing, either with our barcodes kit or with barcodes that you design.
          Connect with Epicentre: Facebook | Twitter

          Comment


          • #6
            do you know when will the Ribo-zero rRNA kit will be available for plant RNA?

            Comment


            • #7
              Answered via PM.
              Connect with Epicentre: Facebook | Twitter

              Comment


              • #8
                Can you PM me the ribo-zero for plant info too?
                Thanks!

                Comment


                • #9
                  I don't think I can PM you as yet, since you're a new user. However, we expect the plant kits to be released early next year. If you're interested in beta-testing the kits, please contact me using the e-mail link in my profile.
                  Connect with Epicentre: Facebook | Twitter

                  Comment


                  • #10
                    ScriptSeq kit ia supposed to produce adaptor-tagged libraries from as little as 50 ng of rRNA-depleted mRNA. My question is addressed to the the vendor (epibio?) as well as to the experienced users.
                    I produced some libraries using 200 ng rRNA-depleted RNA as a starting material. After 10 cycles of PCR amplification, recommended by Epicentre, DNA is barely detectable by spectrophotometry (concentration less than 50 ng/ul) What is the expected yield of double-stranded DNA (final product)? Is it normal to have such low yield, or something have gone wrong, or else my spec is just out of order?

                    Comment


                    • #11
                      50 ng/ul is plenty! One ng of library is sufficient for an entire flow cell (~500 million reads on a HiSeq).

                      Comment


                      • #12
                        Thanks HESmith!
                        I have no experience with those libraries; neither do other people in the lab. This would be our first RNAseq project , thus I have been quite nervous about it .

                        rRNA removal kit of Epicentre worked pretty good, by the way. qPCR shown 78,000-fold reduction of rRNA amplicon signal.
                        Last edited by arabidopsis; 02-24-2011, 09:17 AM.

                        Comment


                        • #13
                          @ arabidopsis: Assuming you had 50 ul after PCR, that should be plenty, as HESmith said. For the kit QC, we use 100 ng of poly(A)+ RNA as input, and obtain around 150 ng of the cDNA library.
                          Connect with Epicentre: Facebook | Twitter

                          Comment


                          • #14
                            @epibio: have you had any feedback from anyone using the mRNA-seq kits for 454? Are you able to point me to anywhere that would have this info? Thanks.

                            Comment


                            • #15
                              We have not heard from any customers using the ScriptSeq Titanium-compatible kit. Most of our development efforts have focused on the Illumina platform, but the Titanium-compatible kit is very similar to the Illumina-compatible one, with the exception of adaptor sequences, and should perform equivalently.
                              Connect with Epicentre: Facebook | Twitter

                              Comment

                              Latest Articles

                              Collapse

                              • seqadmin
                                Strategies for Sequencing Challenging Samples
                                by seqadmin


                                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                                03-22-2024, 06:39 AM
                              • seqadmin
                                Techniques and Challenges in Conservation Genomics
                                by seqadmin



                                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                                Avian Conservation
                                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                                03-08-2024, 10:41 AM

                              ad_right_rmr

                              Collapse

                              News

                              Collapse

                              Topics Statistics Last Post
                              Started by seqadmin, Yesterday, 06:37 PM
                              0 responses
                              8 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, Yesterday, 06:07 PM
                              0 responses
                              8 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, 03-22-2024, 10:03 AM
                              0 responses
                              49 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, 03-21-2024, 07:32 AM
                              0 responses
                              66 views
                              0 likes
                              Last Post seqadmin  
                              Working...
                              X