Hi,
Since ~ 2 months we are getting erratic cluster numbers on our GAs (cBot) even if we check the libraries with RT-PCR. Before we were using 7pM (~300.000clusters /tile) and were getting reproducible, nice results. Now a 7pM sample might result in more than 900.000/mm2 clusters. Also single lanes might totally fail without obvious reasons (not even for techsupport).
I just saw a recent similar thread, but wanted to ask more specific if other/more people are experiencing the same problems.
Thanks
Andi
Since ~ 2 months we are getting erratic cluster numbers on our GAs (cBot) even if we check the libraries with RT-PCR. Before we were using 7pM (~300.000clusters /tile) and were getting reproducible, nice results. Now a 7pM sample might result in more than 900.000/mm2 clusters. Also single lanes might totally fail without obvious reasons (not even for techsupport).
I just saw a recent similar thread, but wanted to ask more specific if other/more people are experiencing the same problems.
Thanks
Andi
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