Hi everyone,
today I tried shearing of a bacterial genome (1,7 Mb). I checked DNA integrity previously and it looks fine with no degradation. I used our standard settings for 550 bp fragment size. Attached you can find the Bioanalyzer results running a DNA 1000 Chip.
The green line is normal human genomic DNA. The other two colors are the bacterial samples, respectively.
I expected to get a peak around the target size or to get no peak because of total fragmentation. But I get two peaks. I get only a small peak in the bacterial samples for the targeted size ans I see a peak around the lower marker indicating a total fragmentation.
Does anyone has some suggestions to optimize shearing of bacterial DNA around 1,7 Mb? Does anyone has suggestions about these results.
Thanks a lot
Elisabeth
today I tried shearing of a bacterial genome (1,7 Mb). I checked DNA integrity previously and it looks fine with no degradation. I used our standard settings for 550 bp fragment size. Attached you can find the Bioanalyzer results running a DNA 1000 Chip.
The green line is normal human genomic DNA. The other two colors are the bacterial samples, respectively.
I expected to get a peak around the target size or to get no peak because of total fragmentation. But I get two peaks. I get only a small peak in the bacterial samples for the targeted size ans I see a peak around the lower marker indicating a total fragmentation.
Does anyone has some suggestions to optimize shearing of bacterial DNA around 1,7 Mb? Does anyone has suggestions about these results.
Thanks a lot
Elisabeth
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