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Old 06-15-2018, 08:14 PM   #1
Zero/1
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Location: San Diego

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Default How to resuspend Ampure beads which fail to resuspend?

I drammatically overdried my Ampure beads, now even pipetting over and over I cannot resuspend them: the lie at the bottom of the tube in large clumps. Any idea? I am afraid I cannot mess this experiment ;(
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Old 06-16-2018, 06:08 PM   #2
luc
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Give it more time and vortex once in a while. They will resuspend in the end.
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Old 06-18-2018, 05:51 AM   #3
r.rosati
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I'm late to the party, but alternatively, wash with ethanol again and vortex well. The ethanol should remove most of the air trapped in the precipitate, and at that point you could dry again and resuspend.

If you tried luc's suggestion, I'd be happy to know if it worked out too!
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Old 06-18-2018, 05:54 PM   #4
Meyana
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I agree on just incubating longer - I put my samples on the thermomixer with intermittent mixing until they dissolve. It works fine
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