Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • stringtie error: GFF not recognized

    Hello,
    I'm trying to run stringtie with a reference genome, and I'm getting the following error:

    WARNING: no reference transcripts were found for the genomic sequences where reads were mapped!
    Please make sure the -G annotation file uses the same naming convention for the genome sequences.

    Similar posts indicate that there is some inconsistency between the reference genome fasta file that was used in the hisat2 step and the gff3 or gtf file that is used for stringtie. I can't see any inconsistency in my case. I'm stumped. I really, really hope someone can help!

    my stringtie command:
    Code:
    stringtie 585_10CR_1.bam -p 8 -B -G /nfs0/ROOTS/Vining_Lab/GENOMES/Mlong585/Mlong_CMEN585_v3.01/Mlong585_v3.01.gff -o 585_10CR_1.gtf
    The reference genome fasta headers look like this:
    Code:
    >scaffold_1_length_46699537
    >scaffold_2_length_45526029
    >scaffold_3_length_45460755
    >scaffold_4_length_44284954
    >scaffold_5_length_43231337

    The first few lines of the gff look like this:
    Code:
    ##gff-version 3
    scaffold1_length_46699537       .       contig  1       46699537        .       .       .       ID=scaffold1_length_46699537;Name=scaffold1_length_46699537
    scaffold1_length_46699537       maker   gene    1701    4304    .       -       .       ID=Mlong585_00001;Name=Mlong585_00001;Alias=augustus-scaffold1_length_46699537-processed-gene-0.17;
    scaffold1_length_46699537       maker   mRNA    1701    4304    .       -       .       ID=Mlong585_00001-RA;Parent=Mlong585_00001;Name=Mlong585_00001-RA;Alias=augustus-scaffold1_length_46699537-processed-gene-0.17-mRNA-1;_AED=1.00
    scaffold1_length_46699537       maker   exon    1701    3837    .       -       .       ID=Mlong585_00001-RA:exon:78;Parent=Mlong585_00001-RA;
    scaffold1_length_46699537       maker   CDS     1701    3837    .       -       1       ID=Mlong585_00001-RA:cds;Parent=Mlong585_00001-RA;
    scaffold1_length_46699537       maker   exon    4012    4097    .       -       .       ID=Mlong585_00001-RA:exon:79;Parent=Mlong585_00001-RA;
    scaffold1_length_46699537       maker   CDS     4012    4097    .       -       0       ID=Mlong585_00001-RA:cds;Parent=Mlong585_00001-RA;
    scaffold1_length_46699537       maker   CDS     4173    4304    .       -       0       ID=Mlong585_00001-RA:cds;Parent=Mlong585_00001-RA;
    scaffold1_length_46699537       maker   exon    4173    4304    .       -       .       ID=Mlong585_00001-RA:exon:80;Parent=Mlong585_00001-RA;
    scaffold1_length_46699537       maker   gene    4819    8990    .       +       .       ID=Mlong585_00002;Name=Mlong585_00002;Alias=snap-scaffold1_length_46699537-processed-gene-0.25;
    scaffold1_length_46699537       maker   mRNA    4819    8990    .       +       .       ID=Mlong585_00002-RA;Parent=Mlong585_00002;Name=Mlong585_00002-RA;Alias=snap-scaffold1_length_46699537-processed-gene-0.25-mRNA-1;_AED=1.00;_QI
    scaffold1_length_46699537       maker   exon    4819    6558    .       +       .       ID=Mlong585_00002-RA:exon:48;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     4819    6558    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     7278    7432    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    7278    7432    .       +       .       ID=Mlong585_00002-RA:exon:49;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     7462    7531    .       +       1       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    7462    7531    .       +       .       ID=Mlong585_00002-RA:exon:50;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     7567    7629    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    7567    7629    .       +       .       ID=Mlong585_00002-RA:exon:51;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     7639    7974    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    7639    7974    .       +       .       ID=Mlong585_00002-RA:exon:52;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     8000    8056    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    8000    8056    .       +       .       ID=Mlong585_00002-RA:exon:53;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     8800    8856    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    8800    8856    .       +       .       ID=Mlong585_00002-RA:exon:54;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   CDS     8958    8990    .       +       0       ID=Mlong585_00002-RA:cds;Parent=Mlong585_00002-RA;
    scaffold1_length_46699537       maker   exon    8958    8990    .       +       .       ID=Mlong585_00002-RA:exon:55;Parent=Mlong585_00002-RA

  • #2
    Hi, I ran into the same error recently. Fortunately was able to fix. My gtf file looks the same, however the genome fasta had extra information in the name. For example, your fasta file should also have the SAME name as the gtf/gff file "scaffold1_length_46699537". Anything before or after will give you that error. I changes the fasta name to match the gff file and it worked!
    Good luck!

    Comment


    • #3
      Could you please explain in detail that how did you solved that issue?
      I mean how did you changed the fasta name to match the gff file.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM
      • seqadmin
        The Impact of AI in Genomic Medicine
        by seqadmin



        Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
        02-26-2024, 02:07 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-14-2024, 06:13 AM
      0 responses
      32 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-08-2024, 08:03 AM
      0 responses
      72 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-07-2024, 08:13 AM
      0 responses
      80 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-06-2024, 09:51 AM
      0 responses
      68 views
      0 likes
      Last Post seqadmin  
      Working...
      X