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Old 01-06-2020, 03:16 PM   #1
aferng
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Location: San Diego

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Default 3' Profile Bias using Illumina Next Seq

I was hoping to get some help regarding this issue Ė
Iím currently running the Truseq stranded mRNA library prep protocol with the nextseq for the purpose of generating a RNAseq library of cell lines.

What Iíve been seeing with my last several runs was a 3í bias for certain genes, but not for others. In addition to that Iím also seeing muted expression profiles across the gene and uneven expression across the exons.

Last edited by aferng; 01-24-2020 at 01:34 PM.
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