Hi, currently doing genome assemblies on 2 very similar samples.
- 1 assembled brilliantly very quickly.
- 1 is highly fragmented
Any reason why one sample should behave so differently from another? - same sequence (Illumina HiSeq), same heterozygosity and repeat content, both screened for contaminants, both collected together, FastQC very similar for both, same assembly methodology, adapter trimmed.
Thoughts:
- adapters in the middle of reads?
- could a virus have inserted itself?
Any comments welcomed.
- 1 assembled brilliantly very quickly.
- 1 is highly fragmented
Any reason why one sample should behave so differently from another? - same sequence (Illumina HiSeq), same heterozygosity and repeat content, both screened for contaminants, both collected together, FastQC very similar for both, same assembly methodology, adapter trimmed.
Thoughts:
- adapters in the middle of reads?
- could a virus have inserted itself?
Any comments welcomed.
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