Hi,
Does anyone known where to find information about the following tophat options:
--solexa-quals
--solexa1.3-quals
I have to align fastq reads (100 nt) issued from a “Hi-Seq 2000” on a reference genome. The data analysis pipeline is HiSeq Control Soft. v. 1.1.37.8 RTA 1.7.48 CASAVA 1.7. So, I'm not sure that the option solexa-quals is the good one in my case ("TopHat was designed to work with reads produced by the Illumina Genome Analyzer").
Thank you,
S.
Does anyone known where to find information about the following tophat options:
--solexa-quals
--solexa1.3-quals
I have to align fastq reads (100 nt) issued from a “Hi-Seq 2000” on a reference genome. The data analysis pipeline is HiSeq Control Soft. v. 1.1.37.8 RTA 1.7.48 CASAVA 1.7. So, I'm not sure that the option solexa-quals is the good one in my case ("TopHat was designed to work with reads produced by the Illumina Genome Analyzer").
Thank you,
S.
Comment