Originally posted by whw
View Post
Might work to perform normalization on the cDNA pre-ligation. Then re-synthesizing 2nd strands. Then blunting/ligation.
The problem there would be that reverse transcription in the TruSeq is designed to work on a much lower amount of RNA (after removal of rRNA). So there might not be enough reagent to convert all that additional RNA.
I guess you could generate cDNA using some other kit. Normalize, then enter TruSeq construction. At that point you might as well use the cheaper DNA TruSeq kit, though.
--
Phillip
Comment