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  • Stranded TruSeq

    Has anyone adapted the second strand cDNA step of the Illumina TruSeq RNA protocol to generate stranded libraries by the dUTP method?
    Did it work?

  • #2
    Originally posted by TonyBrooks View Post
    Has anyone adapted the second strand cDNA step of the Illumina TruSeq RNA protocol to generate stranded libraries by the dUTP method?
    Did it work?
    Looks like "Treebeard" got it to work. Also posted his protocol (note presumes you use the v1 manual, I think).

    --
    Phillip

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