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RNA-Seq: iReckon: Simultaneous isoform discovery and abundance estimation from RNA-se Newsbot! Literature Watch 0 12-04-2012 02:00 AM

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Old 07-08-2016, 10:51 AM   #1
rajesh1989
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Default how many replicates are required for discovery of novel isoform using RNA-seq

Hello everyone,

can I use RNA-seq data from 3 tissues each having 3,3,2 biological replicates and NO technical replicates to identify novel transcripts using cufflinks RABT based assembly?

if not then how many replicates do i need? Thank You.
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Old 07-08-2016, 11:24 AM   #2
GenoMax
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For reference cross-posted: https://www.biostars.org/p/200725/

If is ok to cross-post here.
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