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Old 10-03-2014, 06:47 PM   #1
Location: Canada, Quebec

Join Date: Apr 2012
Posts: 53
Default How to correct a ref seq using Illumina

Dears colleagues,

Last year, I made the assembly of a complete bacterial genome using 454 sequencing reads. However, as you probably know, 454 technology is prone to frameshift. Consequently, I sequenced the strain DNA using Illumina MiSeq. Is there a software to correct reference genome (in fasta) with illumina reads? I know there are iCORN and iCORN 2, unfortunately I have experimented some problems with them.


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Old 10-06-2014, 12:13 PM   #2
Location: Oslo, Norway

Join Date: Nov 2008
Posts: 415

Have a look at pilon:
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