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The NEXTflex™ 18S ITS Amplicon-Seq Kit simplifies the preparation of multiplexed amplicon libraries spanning the hypervariable Internal Transcribed Spacer (ITS) region of eukaryotic 18S ribosomal RNA (rRNA) genes for identification of uncultured fungal and micro-eukaryotic organisms. Up to 384 multiplexing barcodes are available with this kit, to greatly reduce the cost of 18S NGS studies on the Illumina® MiSeq platform. Libraries prepared using this kit are compatible with regular Illumina sequencing primers.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM