Originally posted by lapensee
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"Add 50 ul cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin and pipette up and down 3 times.
Incubate on ice for 3 minutes.
Wash out lysis with 1 ml of cold ATAC-RSB containing 0.1% Tween-20 but NO NP40 or digitonin and invert tube 3 times to mix"
Does this mean I need to removed the previous 50 uL and wash with cold ATAC-RSB?
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