SEQanswers

Go Back   SEQanswers > Applications Forums > Epigenetics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Reply from NEB re: ssDNA library prep RNAarecrazy Epigenetics 0 09-03-2014 08:55 AM
Problems getting results using Illumina / NEB kit for small RNA library prep. Martin Mikkelsen Sample Prep / Library Generation 1 11-26-2013 12:56 AM
using NEB kit to make libraries for Illumina MiSeq mkalive73 Illumina/Solexa 3 10-01-2013 11:13 AM
Indexing ChIPseq libraries using Illumina's TruSeq and ChIPseq kits Alex Clop Epigenetics 6 11-08-2012 12:07 PM
NEB lib prep kit BIG_SNP Illumina/Solexa 0 08-20-2009 03:34 PM

Reply
 
Thread Tools
Old 09-17-2014, 07:20 PM   #1
BioGenomics
Member
 
Location: Belgium

Join Date: Apr 2009
Posts: 24
Default ChIPSeq lilbrary prep for Miseq: NEB or TruSeq ?

Hi all,

is there a consensus on using NEB or Illumina TruSeq ChipSeq lib prep kits ? I assume the NEB is way cheaper anyway, but seemingly had better performance from the latest posts in 2013? I would use it for ChIPSeq analyses in bacteria. What's the best read length/config (50bp SE or longer 100bp PE ?). Is v2 MiSeq still good enough (v3 reagents waste of money for such short frags I assume)

thanks !

Greg
BioGenomics is offline   Reply With Quote
Reply

Tags
chip seq, neb, truseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:46 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO