Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Trinity genome guided error

    Hello,
    Getting this error after running for many hrs:
    Please anybody got into same problem?

    #############################################################################
    This was the command:

    Trinity --genome_guided_bam accepted_hits.sorted.bam --genome_guided_max_intron 10000 --max_memory 60G --CPU 30
    ##############################################################################


    ----------------------------------------------------------------------------------
    -------------- Trinity Phase 1: Clustering of RNA-Seq Reads ---------------------
    ----------------------------------------------------------------------------------

    Wednesday, April 22, 2015: 16:51:14 CMD: find Dir_* -name '*reads' > read_files.list
    Wednesday, April 22, 2015: 16:51:14 CMD: /home/usr/bin/trinityrnaseq-2.0.6/util/support_scripts/GG_write_trinity_cmds.pl --reads_list_file read_files.list --CPU 1 --max_memory 1G --full_cleanup --seqType fa --trinity_complete > trinity_GG.cmds

    ####################################################################################
    #
    # usage: /home/usr/bin/trinityrnaseq-2.0.6/util/support_scripts/GG_write_trinity_cmds.pl --reads_list_file <string> [Trinity params]
    #
    # Required:
    #
    # --reads_list_file <string> file containing list of filenames corresponding
    # to the reads.fasta
    #
    #
    #####################################################################################


    Error, cmd: /home/usr/bin/trinityrnaseq-2.0.6/util/support_scripts/GG_write_trinity_cmds.pl --reads_list_file read_files.list --CPU 1 --max_memory 1G --full_cleanup --seqType fa --trinity_complete > trinity_GG.cmds died with ret 65280 at /home/usr/bin/trinityrnaseq-2.0.6/Trinity line 2116.


    Thanks
    Sandesh

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    Today, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 07:17 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
20 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-29-2024, 10:49 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Working...
X